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Homocysteine pre-treatment increases redox capacity in both endothelial and tumor cells
Elena Díaz-Santiago1, Luis Rodríguez-Caso1, Casimiro Cárdenas1,2
1a Departamento de Biología Molecular y Bioquímica , Facultad de Ciencias, and IBIMA (Biomedical Research Institute of Málaga), Universidad de Málaga , Andalucía Tech , Spain.
Objective:
We studied the modulatory effects of homocysteine pre-treatment on the disulfide reduction capacity of tumor and endothelial cells.
Methods:
Human MDA-MB-231 breast carcinoma and bovine aorta endothelial cells were pre-treated for 1-24 hours with 0.5-5 mM homocysteine or homocysteine thiolactone. After washing to eliminate any rest of homocysteine or homocysteine thiolactone, cell redox capacity was determined by using a method for measuring disulfide reduction.
Results:
Homocysteine pre-treatments for 1-4 hours at a concentration of 0.5-5 mM increase the disulfide reduction capacity of both tumor and endothelial cells. This effect cannot be fully mimicked by either cysteine or homocysteine thiolactone pre-treatments of tumor cells.
Discussion:
Taken together, our data suggest that homocysteine can behave as an anti-oxidant agent by increasing the anti-oxidant capacity of tumor and endothelial cells.
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