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Updated: Mar 21, 2026

A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
Coevolution analysis of Hepatitis C virus genome to identify the structural and functional dependency network of
Raphaël Champeimont1, Elodie Laine1, Shuang-Wei Hu1
1Sorbonne Universités, UPMC-Univ P6, CNRS, Laboratoire de Biologie Computationnelle et Quantitative - UMR 7238, 15 rue de l'Ecole de Médecine, 75006 Paris, France.
Abstract:
A novel computational approach of coevolution analysis allowed us to reconstruct the protein-protein interaction network of the Hepatitis C Virus (HCV) at the residue resolution. For the first time, coevolution analysis of an entire viral genome was realized, based on a limited set of protein sequences with high sequence identity within genotypes. The identified coevolving residues constitute highly relevant predictions of protein-protein interactions for further experimental identification of HCV protein complexes. The method can be used to analyse other viral genomes and to predict the associated protein interaction networks.
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