Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

5.4K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.4K
MicroRNAs01:22

MicroRNAs

4.2K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hyperglycemia-triggered STAT3 activation drives thromboinflammation via NETs formation to promote hemorrhagic transformation after stroke.

International immunopharmacology·2026
Same author

Comparative analysis of neuropsychological impairments between Cushing's disease and non-functioning pituitary adenoma patients: the roles of HPA axis hormones and inflammatory markers in a prospective cohort study.

Endokrynologia Polska·2026
Same author

Comprehensive Pharmacokinetics of the Marine-Derived PDE4 Inhibitor LY104 and Its Major Metabolite M1 in Rats: A Validated LC-MS/MS Method with Sex Comparison, Multiple-Dose, Protein Binding, Metabolic Stability, and Excretion Studies.

Marine drugs·2026
Same author

Vagus nerve as the hub in peripheral immune-inflammatory response: implications for related therapeutic drugs.

Molecular biology reports·2026
Same author

Dicaffeoylquinic acid alleviates alcoholic liver disease by targeting PLA2G4B and inhibiting the MAPK signaling pathway.

Frontiers in pharmacology·2026
Same author

Licochalcone B alleviates pulmonary vascular remodeling via inhibiting Furin/TGF-β1 in pulmonary hypertension.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026

Related Experiment Video

Updated: Mar 21, 2026

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer
09:40

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

3.3K

Oncological miR-182-3p, a Novel Smooth Muscle Cell Phenotype Modulator, Evidences From Model Rats and Patients.

Lan Sun1, Yongyi Bai2, Rui Zhao2

  • 1From the State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China (L.S., R.Z., G.H., W.Z., Y.C., G.D.); Beijing Key Laboratory of Drug Targets Identification and Drug Screening, Beijing, China (L.S., R.Z., G.H., W.Z., Y.C., G.D.); Department of Geriatric Cardiology, Chinese PLA General Hospital, Beijing, China (Y.B., R.C., P.Y.); Department of Cardiology, Huashan Hospital, Fudan University, Shanghai, China (T.S.); and Department of Neurosurgery, PLA General Hospital, Haidian District, Beijing, China (F.W.). sunhanxing2005@imm.ac.cn duguanhua_imm@sina.com yeping301@sina.com.

Arteriosclerosis, Thrombosis, and Vascular Biology
|May 21, 2016
PubMed
Summary

Asymmetrical dimethylarginine promotes vascular remodeling by downregulating miR-182-3p, which targets MYADM. This microRNA (miRNA) pathway influences smooth muscle cell phenotype and atherosclerotic lesion formation.

Keywords:
asymmetrical dimethylarginineatherosclerosismicroRNAmyeloid-associated differentiation markersmooth muscle cellvascular remodeling

More Related Videos

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro
08:28

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

Published on: February 15, 2022

4.4K
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
11:42

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

9.9K

Related Experiment Videos

Last Updated: Mar 21, 2026

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer
09:40

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

3.3K
Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro
08:28

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

Published on: February 15, 2022

4.4K
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
11:42

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

9.9K

Area of Science:

  • Vascular Biology
  • Molecular Medicine
  • MicroRNA Therapeutics

Background:

  • Vascular smooth muscle cell (VSMC) phenotype switching is central to vascular remodeling and atherosclerotic diseases.
  • Asymmetrical dimethylarginine (ADMA) is increasingly linked to vascular remodeling pathologies.
  • MicroRNAs (miRNAs) are emerging regulators of VSMC phenotype and vascular disease.

Purpose of the Study:

  • To investigate the hypothesis that ADMA induces VSMC phenotypic change through a miRNA-dependent mechanism.
  • To identify specific miRNAs and their targets involved in ADMA-mediated vascular remodeling.
  • To explore the therapeutic potential of targeting this miRNA pathway in vascular disease.

Main Methods:

  • Microarray analysis to identify differentially expressed miRNAs in ADMA-treated human aortic SMCs.
  • Validation of miR-182-3p and its target gene, MYADM, using molecular biology techniques.
  • In vitro studies assessing SMC proliferation, migration, and gene expression.
  • In vivo studies using a rat carotid artery balloon injury model to evaluate neointimal formation.
  • Analysis of patient atherosclerotic lesions for miRNA and MYADM expression levels.

Main Results:

  • ADMA treatment led to downregulation of miR-182-3p in human aortic SMCs.
  • MYADM was identified as a direct downstream target of miR-182-3p.
  • miR-182-3p downregulation promoted SMC proliferation and migration via the ERK/MAPK pathway, while MYADM inhibition reversed this.
  • In vivo, miR-182-3p suppressed neointimal formation, whereas its inhibition promoted it.
  • Human atherosclerotic lesions showed inverse correlation between miR-182-3p and MYADM expression.

Conclusions:

  • miR-182-3p acts as a novel modulator of VSMC phenotype by targeting MYADM.
  • The miR-182-3p/MYADM axis represents a potential therapeutic target for vascular remodeling and atherosclerosis.
  • ADMA-induced vascular remodeling is mediated, at least in part, by the suppression of miR-182-3p.