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The incC Sequence Is Required for R27 Plasmid Stability.

Eleonora Tassinari¹, Sonia Aznar¹, Imanol Urcola¹

¹Institut de Bioenginyeria de Catalunya Barcelona, Spain.

Frontiers in Microbiology

|May 21, 2016

Summary

Researchers identified a crucial DNA sequence, incC, essential for the stability of IncHI1 plasmids like R27. Disrupting this sequence significantly reduces plasmid stability, offering a potential new strategy against antimicrobial resistance.

Keywords:

E protein IncHI1 plasmids antimicrobial resistance incC plasmid R27 plasmid stability

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Area of Science:

Microbiology
Molecular Biology
Genetics

Background:

IncHI plasmids are key drivers of antimicrobial resistance in Enterobacteriaceae.
These plasmids, including the R27 archetype, exhibit high stability within their bacterial hosts.
A significant portion of R27's open reading frames (ORFs) lack known functions.

Purpose of the Study:

To identify non-essential DNA sequences in R27 responsible for plasmid stability.
To investigate novel plasmid functions contributing to stability.

Main Methods:

Deletion analysis of non-essential R27 DNA sequences.
Assessing the impact of deletions on plasmid stability in Escherichia coli and Salmonella.

Main Results:

Identification of a short DNA sequence, designated incC, as essential for R27 stability.
The incC region contains repeats and is targeted by the R27 E protein.
Deletion of incC drastically reduced R27 stability, particularly in Salmonella.

Conclusions:

The incC sequence and its interaction with the R27 E protein are critical for IncHI1 plasmid stability.
Targeting the incC-E protein interaction presents a potential novel strategy to combat antimicrobial resistance.