Related Experiment Video
Updated: Mar 20, 2026

Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases
Published on: December 26, 2011
EndoSd: an IgG glycan hydrolyzing enzyme in Streptococcus dysgalactiae subspecies dysgalactiae
Azadeh Shadnezhad1, Andreas Naegeli1, Jonathan Sjögren1,2
1Department of Clinical Sciences, Division of Infection Medicine, Lund University, Biomedical Center B14, SE-22184 Lund, Sweden.
Aim:
The aim of this study was to identify and characterize EndoS-like enzymes in Streptococcus dysgalactiae subspecies dysgalactiae (SDSD).
Materials & Methods:
PCR, DNA sequencing, recombinant protein expression, lectin blot, ultra high performance liquid chromatography analysis and a chitinase assay were used to identify ndoS-like genes and characterize EndoSd.
Results:
EndoSd were found in four SDSD strains. EndoSd hydrolyzes the chitobiose core of the glycan on IgG. The amino acid sequence of EndoSd is 70% identical to EndoS in S. pyogenes, but it has a unique C-terminal sequence. EndoSd secretion is influenced by the carbohydrate composition of the growth medium.
Conclusion:
Our findings indicate that IgG glycan hydrolyzing activity is present in SDSD, and that the activity can be attributed to the here identified enzyme EndoSd.
More Related Videos
12:29Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility
Published on: March 11, 2022
08:37The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides
Published on: November 2, 2021
Related Concept Videos
Oligosaccharide Assembly
Multiple sugar molecules that may or may...
Proteoglycans
Inhibitors of Gram-positive Cell Wall Synthesis
Protein Glycosylation
Glycosylation occurs in...
Glycocalyx and its Functions
Peptidoglycan Synthesis