Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Repulsive interactions between uncharged bilayers. Hydration and fluctuation pressures for monoglycerides.

T J McIntosh1, A D Magid, S A Simon

  • 1Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710.

Biophysical Journal
|May 1, 1989
PubMed
Summary

X-ray diffraction reveals distinct repulsive pressures in lipid bilayers. Hydration pressure dominates in solid phase monoelaidin bilayers, while liquid-crystalline monocaprylin bilayers exhibit both hydration and fluctuation pressures, leading to greater water imbibement.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multisensory Processing of Gustatory Stimuli.

Chemosensory perception·2015
Same author

Gustatory and reward brain circuits in the control of food intake.

Advances and technical standards in neurosurgery·2011
Same author

Compounds from Sichuan and Melegueta peppers activate, covalently and non-covalently, TRPA1 and TRPV1 channels.

British journal of pharmacology·2009
Same author

The gustatory cortex and multisensory integration.

International journal of obesity (2005)·2009
Same author

Evaluation of human fibroblast growth factor 23 (FGF-23) C-terminal and intact enzyme-linked immunosorbent-assays in end-stage renal disease patients.

Clinical laboratory·2009
Same author

X-Ray Diffraction Studies of the Cholera Toxin receptor, G(M1).

Biophysical journal·2009

Area of Science:

  • Physical Chemistry
  • Biophysics
  • Materials Science

Background:

  • Understanding interbilayer forces is crucial for lipid self-assembly and membrane function.
  • Previous studies focused on zwitterionic lipid bilayers, leaving uncharged lipid systems less explored.
  • Lipid bilayer phase (solid vs. liquid-crystalline) significantly impacts their structural and hydration properties.

Purpose of the Study:

  • To quantify pressure-distance relationships for uncharged lipid bilayers in solid (gel) and liquid-crystalline phases.
  • To elucidate the nature and contributions of repulsive pressures (hydration and fluctuation) in these systems.
  • To compare the hydration behavior of monoelaidin (solid phase) and monocaprylin (liquid-crystalline phase) bilayers.

Main Methods:

  • Utilized X-ray diffraction analysis on osmotically stressed aqueous dispersions and multilayers of monoglycerides.

Related Experiment Videos

  • Measured interbilayer pressure as a function of bilayer separation for both solid (monoelaidin) and liquid-crystalline (monocaprylin) phases.
  • Analyzed the decay profiles of repulsive pressures to identify dominant forces at different separations.

    • Main Results:

    • Solid phase monoelaidin bilayers exhibit an exponentially decaying hydration pressure with a decay length of ~1.3 Å, independent of head group charge.
    • Liquid-crystalline monocaprylin bilayers show two distinct pressure regimes: exponential hydration pressure at small separations (3-8 Å) and a distance-dependent fluctuation pressure at larger separations (>8 Å).
    • Monocaprylin bilayers imbibe significantly more water than monoelaidin bilayers due to thermally induced fluctuations.

    Conclusions:

    • Hydration pressure decay length is similar for charged and uncharged lipid bilayers, suggesting head group charge is not critical.
    • Two distinct repulsive pressures, hydration and fluctuation, govern the spacing and water uptake of liquid-crystalline lipid bilayers.
    • Thermally induced fluctuations play a dominant role in the hydration properties of liquid-crystalline lipid bilayers.