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High-throughput production of a stable isotope-labeled peptide library for targeted proteomics using a wheat germ
Nobuaki Takemori1, Ayako Takemori1, Yuki Tanaka2
1Division of Proteomics Research, Proteo-Science Center, Ehime University, Ehime, Japan. takemori@m.ehime-u.ac.jp.
Molecular Biosystems
|May 21, 2016
Abstract:
Quantitative proteomic approaches using selected reaction monitoring (SRM) are currently limited by the difficulty in the preparation of reference standards. In this study, we demonstrat the high-throughput production of a reference peptide library using a wheat germ cell-free synthesis system to develop a large-scale SRM assay for targeted proteomics.
