Related Experiment Videos
Calbindin-D 28K in hippocampal organotypic cultures
N Bousez-Dumesnil1, M Thomasset, Y Ben-Ari
1I.N.S.E.R.M. U. 29, Hôpital de Port-Royal, Paris, France.
Brain Research
|May 1, 1989
Abstract:
Slices of hippocampus from 6-day-old rats were cultured for 2-4 weeks using the roller-tube technique. The organization of these explants was studied by immunocytochemical labeling of calbindin-D 28K (CaBP 28K). The development of the CaBP 28K staining was very close to that of the rat hippocampus in vivo with only 3 subpopulations of labeled cells: granule cells and their mossy fibers, pyramidal cells in the subiculum-CA1 zone and interneurons scattered in strata oriens and radiatum.