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Updated: Mar 20, 2026
DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering
Published on: November 9, 2017
Recyclable magnetite nanoparticle coated with cationic polymers for adsorption of DNA
B Rutnakornpituk1,2, T Theppaleak1, M Rutnakornpituk1,2
1a Faculty of Science, Department of Chemistry and Center of Excellence in Biomaterials , Naresuan University , Phitsanulok , Thailand.
Abstract:
Magnetite nanoparticle (MNP) grafted with a cationic copolymer between poly(2-(N,N-diethylamino) ethyl methacrylate) and poly(poly(ethylene glycol) methyl ether methacrylate)) for efficient and recyclable adsorption of 5'-fluorescein-tagged DNA (FAM-dT9) was prepared. MNP having highest degree of positive charge (+32.1 ± 1.9 mV) retained 100% adsorption of FAM-dT9 during eight adsorption-separation-desorption cycles. The MNP having lower degree of positive charge showed a slight decrease in adsorption percentages (94-98% adsorption) after multiple recycling processes. This biocompatible hybrid material with charged surface and magnetic-responsive properties might be applicable for use as a nanosolid support for efficient and facile separation of various bioentities.
