Related Experiment Video
Updated: Mar 20, 2026
Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
Published on: April 25, 2025
Clinical validation of a real-time polymerase chain reaction assay for rapid detection of Acinetobacter baumannii
P Blanco-Lobo1, V González-Galán2, M García-Quintanilla1
1Biomedical Institute of Seville, University Hospital Virgen del Rocío/CSIC/University of Seville, Seville, Spain.
Abstract:
Real-time polymerase chain reaction (PCR)-based approaches have not been assessed in terms of their ability to detect patients colonized by Acinetobacter baumannii during active surveillance. This prospective, double-blind study demonstrated that a real-time PCR assay had high sensitivity (100%) and specificity (91.2%) compared with conventional culture for detecting A. baumannii in 397 active surveillance samples, and provided results within 3h. Receiver-operator curve analyses demonstrated that the technique has diagnostic accuracy of 97.7% (95% confidence interval 96.0-99.3%). This method could facilitate the rapid implementation of infection control measures for preventing the transmission of A. baumannii.
