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Related Experiment Videos

S-100 protein stimulates cellular proliferation.

J R Klein1, D S Hoon, J Nangauyan

  • 1Division of Surgical Oncology, UCLA School of Medicine, Los Angeles, California 90024.

Cancer Immunology, Immunotherapy : CII
|January 1, 1989
PubMed
Summary

S-100 protein (S-100p) acts as a growth factor, stimulating human melanoma cells and peripheral blood lymphocytes (PBL). This calcium-binding protein may function as an autocrine tumor growth factor in melanoma.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Oncology

Background:

  • S-100 protein (S-100p) is a calcium-binding protein found in various cell types, notably abundant in human melanoma cells compared to normal melanocytes.
  • Its elevated presence in melanoma suggests a potential role in tumor progression.

Purpose of the Study:

  • To investigate if S-100 protein functions as a growth factor for human melanoma cells and peripheral blood lymphocytes (PBL).

Main Methods:

  • Purified S-100p from bovine brain and human melanoma cells was added exogenously to cultures of human melanoma cells and PBL.
  • Cell proliferation was assessed using a [3H]dT-uptake assay at varying S-100p concentrations.

Main Results:

  • S-100p significantly stimulated the proliferation of human melanoma cells within a concentration range of 1.95-31.25 micrograms/ml.

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  • Peripheral blood lymphocytes (PBL) also showed maximum proliferation stimulation at 31.25 micrograms/ml of S-100p, with no inhibition observed even at high concentrations.
  • S-100p stimulation of PBL did not necessitate the presence of monocytes/macrophages.

    • Conclusions:

    • S-100 protein exhibits growth-promoting activity for both melanoma cells and PBL, suggesting it can act as an autocrine growth factor for tumors.
    • The findings indicate that S-100p stimulation is not exclusive to a specific cell type, as PBL are also responsive.