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Updated: Sep 21, 2026

Semi-automated Imaging of Tissue-specific Fluorescence in Zebrafish Embryos
Published on: May 17, 2014
In ovo tris(2-butoxyethyl) phosphate concentrations significantly decrease in late incubation after a single exposure
Margaret L Eng1,2, Robert J Letcher3, Tony D Williams1
1Department of Biological Sciences, Simon Fraser University, Burnaby, British Columbia, Canada.
Abstract:
Zebra finch (Taeniopygia guttata) eggs were injected with the organophosphate triester flame retardant tris(2-butoxyethyl) phosphate (TBOEP) at 0 μg/g, 0.01 μg/g, 1 μg/g, 10 μg/g, or 50 μg/g egg. Subsets of high-dose eggs were collected throughout incubation to measure TBOEP, which started declining in late incubation and then decreased rapidly to 28% of injected concentration by hatching. The authors found no effects of TBOEP on survival, growth, or reproduction even at very high doses. Environ Toxicol Chem 2017;36:83-88. © 2016 SETAC.

