A new method for long-term storage of titred microbial standard solutions suitable for microbiologic quality control...

Area of Science:

• Microbiology
• Biotechnology
• Cryobiology

Background:

• Commercial lyophilized microbial standards are costly.
• Lyophilization can reduce microbial cell viability due to freeze-drying stress.
• There is a need for affordable and reliable microbial standards.

Purpose of the Study:

• To develop an inexpensive in-house method for preparing microbial standards.
• To establish a cryoconservation technique for microbial standards.
• To assess the long-term stability of cell titre and viability using the new method.

Main Methods:

• Development of a straightforward protocol for microbial standard preparation.
• Implementation of a cryoconservation strategy for preserved microbial standards.
• Evaluation of cell titre and viability over a 14-month period.

Main Results:

• The in-house method is significantly less expensive than commercial alternatives.
• The cryopreservation technique maintained constant cell titre.
• High cell viability was preserved for the entire 14-month study duration.

Conclusions:

• The developed in-house method provides a cost-effective solution for microbial standard preparation.
• This approach ensures reliable and stable microbial standards for research and diagnostics.
• The cryoconservation method offers a viable alternative to commercial lyophilized standards.