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Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
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Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

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Corrigendum to 'Methods to account for movement and flexibility in cryo-EM data processing' [Methods 100 (2016)

S Rawson1, M G Iadanza2, N A Ranson2

  • 1School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.

Methods (San Diego, Calif.)
|May 23, 2016
PubMed
Summary

No abstract available in PubMed .

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Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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