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Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness
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Immunoselection techniques in hematopoietic stem cell transplantation

Giuseppina Li Pira1, Simone Biagini1, Elisabetta Cicchetti1

  • 1Department of Pediatric Hematology and Oncology, Unit of Immuno-Hematology and Transfusion Medicine, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.

Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis
|May 23, 2016
PubMed

Hematopoietic Stem Cells Transplantation (HSCT) is an effective treatment for hematological and non-hematological diseases. The main challenge in autologous HSCT is purging of malignant cells to prevent relapse. In allogeneic HSCT graft-versus-host disease (GvHD) and opportunistic infections are frequent complications. Two types of graft manipulation have been introduced: the first one in the autologous context aimed at separating malignant cells from hematopoietic stem cells (HSC), and the second one in allogeneic HSCT aimed at reducing the incidence of GvHD and at accelerating immune reconstitution. Here we describe the manipulations used for cell purging in autologous HSCT or for T Cell Depletion (TCD) and T cell selection in allogeneic HSCT. More complex manipulations, requiring a Good Manufacturing Practice (GMP) facility, are briefly mentioned.

Keywords:
Alloreactive cellsCell manipulationGraft-versus-host diseaseHematopoietic stem cellOpportunistic infections

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