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Updated: Mar 20, 2026

Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii
Published on: August 18, 2019
Characterization of microsatellites identified by next-generation sequencing in the Neotropical tree Handroanthus
Eduardo Morillo1, Johanna Buitron1, Ricardo Limongi1
1Instituto Nacional de Investigaciones Agropecuarias (INIAP), Casilla Postal 17-01-340, Quito, Ecuador.
Premise Of The Study:
We developed microsatellite (simple sequence repeat [SSR]) markers in the Neotropical tree Handroanthus billbergii (Bignoniaceae), to be applied in assessment of genetic diversity in this species as a reference for inferring the impact of dry forest fragmentation in Ecuador.
Methods And Results:
Using next-generation sequencing, we detected a total of 26,893 putative SSRs reported here. Using an ABI 3500xl sequencer, we identified and characterized a set of polymorphic markers in 23 individuals belonging to three populations of H. billbergii.
Conclusions:
We report a set of 30 useful SSR markers for H. billbergii and a large list of potential microsatellites for developing new markers for this or related species.
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