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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
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RNA-seq03:21

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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High-throughput sequencing and vaccine design.

F Luciani

    Revue Scientifique Et Technique (International Office of Epizootics)
    |May 25, 2016
    PubMed
    Summary

    Next-generation sequencing (NGS) advances genomic research, enabling better vaccine development against complex pathogens like viruses and bacteria. This technology provides crucial data for understanding pathogen biology and host interactions, leading to improved vaccine design.

    Keywords:
    GenomeProduction de vaccinReponse immunitaireSequencage haut-debitVaccinVirologie

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    Area of Science:

    • Genomics
    • Vaccinology
    • Infectious Disease Research

    Background:

    • Next-generation sequencing (NGS) has revolutionized genome research, revealing unprecedented genomic detail and complexity in various organisms.
    • This increased genomic understanding impacts the study of pathogen biology and host interactions, presenting both challenges and opportunities.

    Purpose of the Study:

    • To review the impact of broad NGS applications on vaccine research.
    • To focus on the implications for viral genomics and vaccine development against challenging pathogens.

    Main Methods:

    • Literature review of recent animal and human studies.
    • Analysis of how NGS technologies influence vaccine design and research strategies.

    Main Results:

    • NGS provides critical data for developing vaccines against complex pathogens, including rapidly mutating RNA viruses and bacteria with intricate host immune interactions.
    • The technology enhances the understanding of pathogen genomes, aiding in the design of more effective vaccines.

    Conclusions:

    • NGS technologies are fundamentally changing vaccine research by providing deeper insights into pathogen genomics.
    • The application of NGS is crucial for overcoming challenges in developing vaccines against difficult-to-target pathogens.