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Biofunctionalization of Magnetic Nanomaterials
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A Facile Approach to Functionalize Cell Membrane-Coated Nanoparticles.

Hao Zhou1, Zhiyuan Fan1, Pelin K Lemons1

  • 11. Department of Materials Science and Engineering, Drexel University, Philadelphia, Pennsylvania, USA;

Theranostics
|May 25, 2016
PubMed
Summary
This summary is machine-generated.

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We developed a facile method to engineer cell membrane-coated nanoparticles (CM-NPs) with enhanced functions. This approach successfully conjugated enzymes onto red blood cell membranes, improving nanoparticle diffusion without compromising circulation time.

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Surface Chemistry

Background:

  • Cell membrane-coated nanoparticles (CM-NPs) offer unique properties but their applications are limited by their inherent functionalities.
  • Developing strategies to impart multi-functionalities to CM-NPs is crucial for expanding their use.
  • Existing methods may alter natural cell membrane properties, affecting nanoparticle performance.

Purpose of the Study:

  • To develop a facile chemical modification strategy for live cell membranes prior to CM-NP fabrication.
  • To enable the conjugation of large bioactive molecules, such as enzymes, onto CM-NPs.
  • To demonstrate that functionalized CM-NPs retain their natural properties while gaining enhanced functionalities.

Main Methods:

  • Utilized a bifunctional linker, succinimidyl-[(N-maleimidopropionamido)-polyethyleneglycol] ester (NHS-PEG-Maleimide), to modify live cell membranes.
Keywords:
Tumor penetrationbiomimeticsdrug delivery.extracellular matrixlymph nodes

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  • Conjugated human recombinant hyaluronidase, PH20 (rHuPH20), onto red blood cell (RBC) membranes using linkers of different molecular weights (425 Da vs. 3400 Da).
  • Fabricated functionalized membranes into RBC membrane-coated nanoparticles (RBCM-NPs) and evaluated their diffusion properties and in vivo circulation time.

    • Main Results:

    • A long PEG linker (3400 Da) was superior for maintaining enzyme activity and minimizing cell membrane alterations compared to a short linker (425 Da).
    • Conjugated rHuPH20 on RBCM-NPs significantly enhanced nanoparticle diffusion in matrix-mimicking gels and cancer cell pericellular matrices.
    • Functionalization with rHuPH20 did not impede the ultra-long blood circulation time of RBCM-NPs, confirmed after chemical group quenching.

    Conclusions:

    • The developed surface engineering approach provides a versatile platform for functionalizing CM-NPs.
    • This method allows for the controlled conjugation of bioactive molecules, enhancing nanoparticle function without sacrificing inherent properties.
    • This strategy holds promise for advancing the application of CM-NPs in various biomedical fields.