Related Experiment Video
Updated: Mar 20, 2026
05:41
Purification of Platelets from Mouse Blood
Published on: May 7, 2019
23.9K
Quantification of platelets obtained by different centrifugation protocols in SHR rats
João Alberto Yazigi Junior1, João Baptista Gomes Dos Santos1, Bruno Rodrigues Xavier1
1Universidade Federal de São Paulo (Unifesp), São Paulo, SP, Brazil.
Revista Brasileira De Ortopedia
|May 25, 2016
Summary
This study found that a double centrifugation method significantly increases platelet concentration in SHR rat blood. The optimal protocol involved two 400 x g centrifugations for enhanced platelet yield.
Area of Science:
- Hematology
- Animal Models
Background:
- Platelet-rich plasma (PRP) is crucial for research and therapeutic applications.
- Standardized methods for obtaining high-quality platelet concentrates from animal models are needed.
Purpose of the Study:
- To determine the most effective centrifugation protocol for maximizing platelet concentration in Spontaneously Hypertensive Rat (SHR) blood.
- To quantify platelet yield using various single and double centrifugation techniques.
Main Methods:
- Forty male SHR rats were used, divided into control (no centrifugation) and experimental groups.
- Experimental groups underwent single or double centrifugation protocols with varying speeds (200-800 x g) and durations.
- Platelet counts were performed on plasma samples obtained via cardiac puncture and processed using Vacutainer tubes with sodium citrate.
Main Results:
- The double centrifugation protocol of 400 x g for 10 minutes followed by another 400 x g for 10 minutes yielded the highest platelet enrichment.
- This optimal method resulted in a mean platelet concentration 11.30 times greater than the control group.
Conclusions:
- Simple and viable techniques using common laboratory materials can achieve high platelet concentrations.
- Double centrifugation is the most effective method for obtaining platelet concentrate from SHR rat blood.
