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Hepatitis

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Hepatitis is an inflammatory condition of the liver most commonly caused by hepatotropic viruses (A–E), though non-infectious causes such as alcohol and drugs also exist.Hepatitis AHepatitis A virus (HAV) is a non-enveloped RNA virus of the Picornaviridae family. It is primarily transmitted via the fecal-oral route, typically through ingestion of contaminated food or water. After ingestion, HAV enters the bloodstream through the oropharynx or intestinal epithelium and reaches the liver.

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Related Experiment Video

Updated: Mar 20, 2026

A Protocol for Analyzing Hepatitis C Virus Replication

A Protocol for Analyzing Hepatitis C Virus Replication

Published on: June 26, 2014

Calsyntenin-1 mediates hepatitis C virus replication.

Zunaira Awan¹, Enoch S E Tay¹, Nicholas S Eyre²

¹Storr Liver Centre, The Westmead Millennium Institute for Medical Research, The University of Sydney at Westmead Hospital, 176 Hawkesbury Rd, Westmead NSW 2145, Australia.

The Journal of General Virology

|May 26, 2016

Summary

Researchers identified calsyntenin-1 as a host factor crucial for hepatitis C virus (HCV) replication. Silencing this protein disrupts the viral lifecycle, highlighting its importance in HCV pathogenesis.

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Last Updated: Mar 20, 2026

A Protocol for Analyzing Hepatitis C Virus Replication

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Published on: June 26, 2014

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors

Published on: July 16, 2012

Area of Science:

Virology
Cell Biology
Molecular Biology

Background:

Hepatitis C virus (HCV) relies heavily on host cell factors for its replication due to its limited protein-coding capacity.
Understanding host-pathogen interactions is key to deciphering viral replication mechanisms and developing antiviral strategies.

Purpose of the Study:

To identify host cell factors involved in the egress of hepatitis C virus (HCV) particles.
To elucidate the role of identified host factors in the HCV replication cycle.

Main Methods:

Utilized SILAC-based proteomics to screen the supernatant of HCV-infected Huh7 cells.
Investigated the function of calsyntenin-1 in intracellular transport and its interaction with viral components.
Employed gene silencing techniques to assess the impact of calsyntenin-1 on HCV replication.

Main Results:

Identified calsyntenin-1, a transmembrane protein, as specifically secreted by HCV-infected cells.
Demonstrated that calsyntenin-1 mediates intracellular transport of endosomes in hepatocytes, similar to its role in neurons.
Showed that calsyntenin-1 contributes to early stages of HCV replication and replication complex formation.
Confirmed that silencing calsyntenin-1 significantly disrupts the HCV replication cycle.

Conclusions:

Calsyntenin-1 is a critical host factor essential for hepatitis C virus replication.
The findings enhance understanding of HCV replication cycle and pathogenesis.
Calsyntenin-1's role may have implications for other viruses utilizing similar host pathways.