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Updated: Mar 20, 2026

A Revised Method for Inducing Secondary Lymphedema in the Hindlimb of Mice
Published on: November 2, 2019
LYMPHA Technique to Prevent Secondary Lower Limb Lymphedema
Francesco Boccardo1, Mario Valenzano2, Sergio Costantini2
1Unit of Lymphatic Surgery, Department of Surgery, IRCCS S. Martino Hospital - IST, National Cancer Institute for Cancer Research, University of Genoa, Genova, Italy. francesco.boccardo@unige.it.
The LYMPHA technique, involving multiple lymphatic-venous anastomoses, shows promise in preventing lower limb lymphedema after inguinofemoral lymphadenectomy for cancer patients.
Area of Science:
- Surgical Oncology
- Lymphedema Research
- Microsurgery
Background:
- Inguinofemoral lymphadenectomy is associated with a high risk of lower limb lymphedema.
- The LYMPHA technique aims to mitigate this risk by performing multiple lymphatic-venous anastomoses (MLVA).
Purpose of the Study:
- To assess the feasibility and safety of the LYMPHA technique.
- To evaluate the effectiveness of LYMPHA in preventing lower limb lymphedema.
Main Methods:
- 11 vulvar cancer and 16 melanoma patients underwent inguinofemoral lymphadenectomy and the LYMPHA technique.
- Blue dye was used to identify lymphatics for MLVA with a saphenous vein collateral branch.
Main Results:
- No lymphocele or infectious complications were observed.
- Only one patient (6.25%) experienced transient edema, and one (9%) had permanent edema.
Conclusions:
- The LYMPHA technique is feasible, safe, and effective in preventing lower limb lymphedema.
- This technique can improve patient quality of life and reduce healthcare costs.
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