Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Differential Impacts of Components of Particulate Matter on Epithelial Cell Toxicity and Immune Responses.

ACS omega·2026
Same author

Heritable single-cell gene expression states shape functional variability in innate immune responses.

bioRxiv : the preprint server for biology·2026
Same author

T-cell-targeted immunotherapy in neurofibromatosis type 2-related vestibular schwannoma: current evidence and future direction.

Brain communications·2026
Same author

Specific Deletion of Interleukin-1 Beta in Microglia Improves Acute Outcome and Modulates Neurogenesis After Ischemic Stroke.

Glia·2026
Same author

Disruption of macrophage cell volume drives inflammatory responses and type I interferon signaling.

The Journal of cell biology·2026
Same author

Domain-specific mechanisms of YAP1 variants in ocular coloboma revealed by in-vitro and organoid studies.

Biochimica et biophysica acta. Molecular basis of disease·2026

Related Experiment Video

Updated: Mar 20, 2026

Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice
09:34

Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice

Published on: June 22, 2016

10.1K

Investigating IL-1β Secretion Using Real-Time Single-Cell Imaging.

Catherine Diamond1,2, James Bagnall1, David G Spiller1

  • 1Faculty of Life Sciences, University of Manchester, AV Hill Building, Oxford Road, Manchester, UK.

Methods in Molecular Biology (Clifton, N.J.)
|May 26, 2016
PubMed
Summary
This summary is machine-generated.

Interleukin (IL)-1β, a key inflammatory mediator, is released from cells via an undefined mechanism. This study developed a novel method to investigate IL-1β secretion at the single-cell level, suggesting membrane permeabilization is involved.

Keywords:
Confocal microscopyIL-1β VenusIL-1β secretionLentiviral transductionReal-time single-cell imaging

More Related Videos

Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin
13:45

Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin

Published on: December 23, 2010

13.3K
Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses
09:43

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses

Published on: March 8, 2024

2.6K

Related Experiment Videos

Last Updated: Mar 20, 2026

Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice
09:34

Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice

Published on: June 22, 2016

10.1K
Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin
13:45

Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin

Published on: December 23, 2010

13.3K
Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses
09:43

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses

Published on: March 8, 2024

2.6K

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Interleukin (IL)-1β is a critical pro-inflammatory cytokine.
  • IL-1β secretion lacks a conventional signal peptide, making its release mechanism unclear.
  • Previous methods limited studies to population dynamics, masking single-cell variations.

Purpose of the Study:

  • To develop a sensitive method for investigating IL-1β secretion at the single-cell level.
  • To explore the mechanisms underlying IL-1β release from cells.
  • To test the hypothesis that IL-1β secretion involves membrane permeabilization.

Main Methods:

  • Development of a novel vector encoding a fluorescently labeled IL-1β.
  • Utilization of real-time single-cell confocal microscopy.
  • Establishment of a protocol for sensitive, single-cell analysis of IL-1β secretion.

Main Results:

  • Achieved enhanced sensitivity in detecting IL-1β release.
  • Enabled real-time observation of IL-1β dynamics in single cells.
  • Provided a platform for investigating IL-1β secretion mechanisms.

Conclusions:

  • The developed protocol offers a sensitive approach to study IL-1β secretion.
  • This method facilitates the investigation of IL-1β release mechanisms.
  • The study provides a foundation for testing hypotheses regarding IL-1β secretion, such as the role of membrane permeabilization.