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Ginsenoside-Rh2 Inhibits Proliferation and Induces Apoptosis of Human Gastric Cancer SGC-7901 Side Population Cells
Jun Qian1, Jing Li, Jian-Guang Jia
1Third Department of Tumor Surgery, First Affiliated Hospital of Bengbu Medical College, Bengbu, China
Objectives:
To observed the effects of ginsenoside -Rh2 (GS-Rh2) on proliferation and apoptosis of side population (SP) human gastric cancer SGC-7901 cells.
Materials And Methods:
SGC-7901 SP and Non-SP cells were sorted by flow cytometry and assessed using the cck-8 method. Expression of apoptosis-related proteins Bax and Bcl-2 of SP before and after the intervention was determined by Western-blotting.
Results:
It was found that the proliferation of SP was significantly faster than that of NSP (<0.05). In addition, GS-Rh2 inhibited proliferation of gastric cancer SP cells, induced cell cycle arrest and cell apoptosis, and changed the expression of BAX/Bcl-2 proteins in a time-dependent and concentration-dependent manner (<0.05).
Conclusions:
With increase of GS-Rh2 dose, GS-Rh2 gradually inhibit the proliferation of SGC-7901 SP cells, which have high proliferation rate, through G1/G0 phase arrest, followed by apoptosis which involves the up-regulation of Bax and the down-regulation of Bcl-2.
Insights
Ginsenoside-Rh2 (GS-Rh2) effectively inhibits gastric cancer stem cell proliferation and induces apoptosis. This natural compound targets SGC-7901 SP cells by arresting their cell cycle and altering key protein expressions.
Area of Science:
- Oncology
- Pharmacology
- Cell Biology
Background:
- Gastric cancer stem cells (SP cells) exhibit high proliferation rates and resistance to conventional therapies.
- Ginsenoside-Rh2 (GS-Rh2) is a promising natural compound derived from ginseng with potential anti-cancer properties.
Purpose of the Study:
- To investigate the effects of GS-Rh2 on the proliferation and apoptosis of side population (SP) human gastric cancer SGC-7901 cells.
- To elucidate the underlying mechanisms of GS-Rh2's action on gastric cancer stem cells.
Main Methods:
- Human gastric cancer SGC-7901 cells were sorted into SP and Non-SP populations using flow cytometry.
- Cell proliferation was assessed using the CCK-8 assay.
- Apoptosis-related protein expression (Bax and Bcl-2) was determined by Western blotting.
Main Results:
- SP cells demonstrated significantly higher proliferation rates compared to Non-SP cells.
- GS-Rh2 treatment inhibited the proliferation of gastric cancer SP cells in a time- and concentration-dependent manner.
- GS-Rh2 induced cell cycle arrest at the G1/G0 phase and promoted apoptosis, evidenced by altered Bax/Bcl-2 protein ratios.
Conclusions:
- GS-Rh2 effectively inhibits the proliferation of highly proliferative SGC-7901 SP cells.
- The anti-cancer effects of GS-Rh2 involve G1/G0 phase arrest and induction of apoptosis.
- GS-Rh2 modulates apoptosis by up-regulating Bax and down-regulating Bcl-2 expression.
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