Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

4.7K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
4.7K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

17.6K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
17.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interference of stress with the somatotropic axis in pigs - lights on new biomarkers.

Scientific reports·2017
Same author

Atomic force microscopy of RNA: State of the art and recent advancements.

Seminars in cell & developmental biology·2017
Same author

Nanoparticular surface-bound PCBs, PCDDs, and PCDFs-a novel class of potentially higher toxic POPs.

Environmental science and pollution research international·2016
Same author

PCDDs, PCDFs, and PCBs co-occurrence in TiO2 nanoparticles.

Environmental science and pollution research international·2015
Same author

Optical imaging beyond the diffraction limit by SNEM: effects of AFM tip modifications with thiol monolayers on imaging quality.

Ultramicroscopy·2014
Same author

Sub-nanometer expansions of redox responsive polymer films monitored by imaging ellipsometry.

Nanoscale·2014

Related Experiment Video

Updated: Mar 20, 2026

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging
09:52

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

Published on: January 31, 2019

12.3K

Imaging and force probing RNA by atomic force microscopy.

Peter Schön1

  • 1NanoBioInterface Chair, Research Center Design and Technology, Saxion University of Applied Sciences, 7500 KB Enschede, The Netherlands; Materials Science and Technology of Polymers, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.

Methods (San Diego, Calif.)
|May 26, 2016
PubMed
Summary

Atomic Force Microscopy (AFM) provides high-resolution structural and dynamic insights into RNA molecules, assemblies, and interactions. This technique is crucial for advancing RNA nanotechnology and understanding biological RNA functions.

Keywords:
AFMForce spectroscopyRNARNA assemblies

More Related Videos

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping
14:13

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Published on: October 24, 2014

12.2K
High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping
08:59

High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping

Published on: March 22, 2024

1.2K

Related Experiment Videos

Last Updated: Mar 20, 2026

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging
09:52

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

Published on: January 31, 2019

12.3K
Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping
14:13

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Published on: October 24, 2014

12.2K
High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping
08:59

High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping

Published on: March 22, 2024

1.2K

Area of Science:

  • Life Sciences
  • Nanotechnology
  • Biophysics

Background:

  • Atomic Force Microscopy (AFM) has revolutionized life sciences over 30 years, enabling label-free visualization and manipulation of biological systems.
  • AFM provides nanometer spatial resolution, making it a routine tool for structural studies of nucleic acids, including single-stranded RNA (ssRNA), double-stranded RNA (dsRNA), and their complexes.

Purpose of the Study:

  • To review the application of AFM in RNA research, covering imaging, force spectroscopy, and probing techniques.
  • To highlight AFM's contribution to understanding RNA structure, interactions, and dynamics for RNA nanotechnology and biological studies.

Main Methods:

  • Description of common AFM modes, including topographic imaging and AFM-based force spectroscopy.
  • Application of AFM for structural analysis of RNA, RNA assemblies, and aggregates in aqueous buffer under physiological conditions.

Main Results:

  • AFM enables detailed topographic imaging of various RNA structures, from single molecules to complex assemblies and aggregates.
  • AFM-based force spectroscopy probes RNA interactions with proteins and ligands, providing insights into biological relevance and dynamics.

Conclusions:

  • AFM is an indispensable tool for structural and dynamic studies of RNA, significantly contributing to RNA research and engineering.
  • The technique offers unique capabilities for exploring RNA-based building blocks in emerging fields like RNA nanotechnology.