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Updated: Mar 20, 2026

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
Published on: October 1, 2014
Trajectories and single-particle tracking data of intracellular vesicles loaded with either SNAP-Crb3A or SNAP-Crb3B
Jan Peter Siebrasse1, Ivona Djuric2, Ulf Schulze2
1Institute of Physical and Theoretical Chemistry, Rheinische Friedrich-Wilhelms University Bonn, Wegelerstraße 12, 53115 Bonn, Germany.
Abstract:
Using a combined approach of pulse chase labeling and single-particle tracking of Crb3A or 3B loaded vesicles we collected trajectories of different vesicle population in living podocyte cells and evaluated statistically their different mobility patterns. Differences in their intracellular mobility and in their directed transport correspond well to the role of Crb3A and 3B in renal plasma membrane sorting (Djuric et al., 2016) [1].
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