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MicroRNA-138 acts as a tumor suppressor in non small cell lung cancer via targeting YAP1

Ling Xiao1,2,3, Hui Zhou4, Xiang-Ping Li1,2

  • 1Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Hunan 410078, P.R. China.

Oncotarget
|May 26, 2016
PubMed

Insights

MicroRNA-138 (miR-138) suppresses non-small cell lung cancer (NSCLC) growth and metastasis by targeting YAP1. Lower miR-138 levels correlate with advanced NSCLC, and restoring miR-138 inhibits cancer cell proliferation and invasion.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • MicroRNA-138 (miR-138) exhibits tumor-suppressive properties in various human cancers.
  • The specific role and regulatory mechanisms of miR-138 in non-small cell lung cancer (NSCLC) require further elucidation.

Purpose of the Study:

  • To investigate the regulatory mechanism of miR-138 in NSCLC.
  • To identify the direct target gene of miR-138 and its role in NSCLC progression.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) for miR-138 expression analysis.
  • Bioinformatic prediction, luciferase reporter assays, and Western blot to identify and validate miR-138 targets.
  • Cell transfection, MTT, Transwell, and wound healing assays to assess cell proliferation, invasion, and migration.

Main Results:

  • miR-138 was significantly downregulated in NSCLC tissues and cell lines, correlating with poor differentiation, advanced stage, and lymph node metastasis.
  • YAP1 was identified as a direct target of miR-138, with its protein levels negatively regulated by miR-138 in NSCLC cells.
  • Overexpression of miR-138 inhibited NSCLC cell growth, invasion, and migration, while YAP1 overexpression partially rescued the suppressive effect on proliferation.
  • YAP1 was upregulated in NSCLC tissues and inversely correlated with miR-138 levels.

Conclusions:

  • miR-138 plays a suppressive role in NSCLC growth and metastasis, at least partly through targeting YAP1.
  • The miR-138/YAP1 axis represents a potential therapeutic target for NSCLC treatment.
Keywords:
Yes-associated protein 1growthmetastasismicroRNA-138non small cell lung cancer

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