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Updated: Mar 20, 2026
Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes
Published on: September 17, 2012
Anopheles darlingi polytene chromosomes: revised maps including newly described inversions and evidence for
Anthony J Cornel1, Katherine K Brisco1, Wanderli P Tadei2
1Mosquito Control Research Laboratory, Department of Entomology and Nematology, University of California, Davis, CA, USA.
Abstract:
Salivary gland polytene chromosomes of 4th instar Anopheles darlingi Root were examined from multiple locations in the Brazilian Amazon. Minor modifications were made to existing polytene photomaps. These included changes to the breakpoint positions of several previously described paracentric inversions and descriptions of four new paracentric inversions, two on the right arm of chromosome 3 and two on the left arm of chromosome 3 that were found in multiple locations. A total of 18 inversions on the X (n = 1) chromosome, chromosome 2 (n = 7) and 3 (n = 11) were scored for 83 individuals from Manaus, Macapá and Porto Velho municipalities. The frequency of 2Ra inversion karyotypes in Manaus shows significant deficiency of heterozygotes (p < 0.0009). No significant linkage disequilibrium was found between inversions on chromosome 2 and 3. We hypothesize that at least two sympatric subpopulations exist within the An. darlingi population at Manaus based on inversion frequencies.
