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Single-Molecule Force Spectroscopy Reveals Multiple Binding Modes between DOPA and Different Rutile Surfaces.

Yiran Li¹, Huanyu Liu¹, Tiankuo Wang¹

¹National Laboratory of Solid State Microstructure, Department of Physics, Nanjing University, 22 Hankou Road, Nanjing, Jiangsu, 210093, China.

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry

|May 26, 2016

Summary

Marine-inspired 3,4-dihydroxyphenylalanine (DOPA) coatings show strong wet adhesion. Single-molecule force spectroscopy reveals DOPA binds to titanium dioxide surfaces via multiple modes, with forces varying based on surface properties.

Keywords:

atomic force microscopy coordination bond strength mussel adhesion single-molecule spectroscopy surface structure

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Area of Science:

Biomaterials science
Surface chemistry
Nanotechnology

Background:

Marine mussels utilize 3,4-dihydroxyphenylalanine (DOPA) for strong underwater adhesion.
DOPA-based materials are developed for wet adhesion applications.
The molecular mechanisms underlying DOPA adhesion are not fully understood.

Purpose of the Study:

To investigate the molecular binding mechanisms of DOPA to well-defined crystal surfaces.
To quantify the adhesion forces between DOPA and rutile titanium dioxide (TiO2) surfaces.
To explore the influence of surface properties on DOPA adhesion.

Main Methods:

Atomic Force Microscopy (AFM)-based single-molecule force spectroscopy (SMFS).
Characterization of DOPA binding forces to different rutile TiO2 surfaces.
Analysis of adhesion force variations based on surface properties.

Main Results:

DOPA exhibits multiple binding modes to chemically defined rutile TiO2 surfaces.
Adhesion forces between DOPA and TiO2 surfaces range from 40-800 pN at a pulling speed of 1000 nm/s.
DOPA adhesion forces are highly dependent on the specific surface properties and local chemical environment.

Conclusions:

The local chemical environment significantly influences DOPA adhesion.
SMFS is a powerful technique for elucidating the heterogeneous nature of DOPA adhesion.
Understanding these molecular interactions is crucial for designing advanced DOPA-based adhesives.