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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

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Updated: Mar 20, 2026

Field Identification of Matricaria chamomilla using a Portable qPCR System

Field Identification of Matricaria chamomilla using a Portable qPCR System

Published on: October 10, 2020

Authentication of Herbal Supplements Using Next-Generation Sequencing.

Natalia V Ivanova¹, Maria L Kuzmina¹, Thomas W A Braukmann¹

¹Centre for Biodiversity Genomics, Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, Canada.

|May 27, 2016

Summary

This summary is machine-generated.

DNA testing for herbal supplements is challenging due to degraded DNA and complex biological interactions. A combined DNA and bioactive component analysis is recommended for accurate quality control.

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Area of Science:

Pharmacognosy
Molecular Biology
Food Science

Background:

DNA-based testing is increasingly used for food authentication, but its application to herbal supplements is debated.
Challenges exist in accurately identifying plant species and detecting contamination in complex herbal matrices.

Purpose of the Study:

To evaluate the efficacy of Sanger and Next-Generation Sequencing (NGS) for authenticating herbal supplements.
To assess the presence of active medicinal components in Ginkgo supplements using HPLC-MS.
To propose an improved quality control strategy for herbal products.

Main Methods:

Utilized Sanger and NGS for DNA sequencing of fifteen herbal supplements from five medicinal plants.
Optimized DNA extraction and sequencing protocols, including Internal Amplification Control and negative controls.
Employed High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) to analyze active compounds in Ginkgo supplements.

Main Results:

Sanger sequencing yielded uninterpretable results due to mixed DNA profiles.
NGS successfully detected manufacturer-listed plant DNA in most dry herb supplements, but DNA degradation was common.
NGS identified diverse fungal DNA, and HPLC-MS confirmed the presence of key medicinal components in Ginkgo supplements despite DNA degradation.

Conclusions:

A synergistic approach combining DNA-based and bioactive component analysis is crucial for herbal supplement quality control, especially for degraded DNA samples.
The developed NGS workflow offers reliable detection of plant and fungal DNA for manufacturers' quality assurance.
Interpreting results requires an interdisciplinary approach considering production processes and biological interactions.