[Study on the Interaction in Resveratrol-Hordein Nanoparticle]

Area of Science:

• Biochemistry
• Spectroscopy
• Materials Science

Background:

• Resveratrol is a polyphenol with potential health benefits.
• Hordein is a major protein found in barley.
• Understanding their interaction is key for developing novel nanoparticles.

Purpose of the Study:

• To elucidate the interaction mechanism between resveratrol and hordein.
• To characterize the formation of resveratrol-hordein nanoparticles.
• To investigate the conformational changes in hordein upon binding with resveratrol.

Main Methods:

• Fluorescence spectroscopy
• UV-Vis spectroscopy
• Fourier-transform infrared (FTIR) spectroscopy
• Differential scanning calorimetry (DSC)
• Synchronous fluorescence spectrometry

Main Results:

• Resveratrol inhibited hordein's fluorescence in a dose-dependent manner, indicating complex formation via a static quenching process.
• Binding constants and number of binding sites were determined.
• Hydrogen bonding and van der Waals forces were identified as the primary interaction forces.
• Resveratrol binding altered hordein's conformation, affecting tryptophan residue hydrophobicity.

Conclusions:

• Resveratrol and hordein form a stable nanoparticle complex.
• The interaction is primarily driven by hydrogen bonding and van der Waals forces.
• Resveratrol significantly influences hordein's structure and properties.