Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Synthetic Biology02:55

Synthetic Biology

5.8K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
5.8K
Next-generation Sequencing03:00

Next-generation Sequencing

100.7K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
100.7K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

21.4K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
21.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RNA Interference: A Natural Immune System of Plants to Counteract Biotic Stressors.

Cells·2019
Same author

Label-free detection of folic acid using a sensitive fluorescent probe based on ovalbumin stabilized copper nanoclusters.

Talanta·2019
Same author

Assessing and predicting changes of the ecosystem service values based on land use/cover change in Ebinur Lake Wetland National Nature Reserve, Xinjiang, China.

The Science of the total environment·2019
Same author

Jasmonate promotes artemisinin biosynthesis by activating the TCP14-ORA complex in <i>Artemisia annua</i>.

Science advances·2019
Same author

[Composition and Predictive Functional Analysis of Rhizosphere Bacterial Communities in Riparian Buffer Strips in the Danjiangkou Reservoir, China].

Huan jing ke xue= Huanjing kexue·2019
Same author

Antibacterial activity and action mechanism of questin from marine <i>Aspergillus flavipes</i> HN4-13 against aquatic pathogen <i>Vibrio harveyi</i>.

3 Biotech·2019

Related Experiment Video

Updated: Mar 20, 2026

Designing a Bio-responsive Robot from DNA Origami
13:32

Designing a Bio-responsive Robot from DNA Origami

Published on: July 8, 2013

22.9K

Designer nanoscale DNA assemblies programmed from the top down.

Rémi Veneziano1, Sakul Ratanalert2, Kaiming Zhang3

  • 1Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Science (New York, N.Y.)
|May 28, 2016
PubMed
Summary
This summary is machine-generated.

Researchers developed a new DNA origami method to autonomously design complex molecular structures based on shape. This top-down strategy bypasses manual base-pairing design, enabling versatile applications.

More Related Videos

Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

15.2K
Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

12.2K

Related Experiment Videos

Last Updated: Mar 20, 2026

Designing a Bio-responsive Robot from DNA Origami
13:32

Designing a Bio-responsive Robot from DNA Origami

Published on: July 8, 2013

22.9K
Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

15.2K
Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

12.2K

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Molecular Engineering

Background:

  • Scaffolded DNA origami enables complex molecular architecture synthesis.
  • Current methods require manual design of Watson-Crick base pairing for target structures.

Purpose of the Study:

  • To develop a general, top-down strategy for autonomous design of DNA architectures based solely on target shape.
  • To overcome the limitations of manual base-pairing design in DNA origami.

Main Methods:

  • Representing objects as closed surfaces rendered as polyhedral networks of parallel DNA duplexes.
  • Utilizing a spanning tree algorithm for complete DNA scaffold routing.
  • Employing asymmetric polymerase chain reaction for assembly production.
  • Verifying structural fidelity using single-particle cryo-electron microscopy.

Main Results:

  • Demonstrated autonomous design of nearly arbitrary DNA architectures based on target shape.
  • Produced stable, monodisperse DNA assemblies with custom scaffold length and sequence.
  • Confirmed high-fidelity 3D structures through cryo-electron microscopy.
  • Showcased long-term stability of DNA assemblies in serum and low-salt buffer.

Conclusions:

  • The developed top-down strategy enables autonomous design of complex DNA nanostructures.
  • The method offers versatility for both biological and nonbiological applications.
  • High-fidelity and stable DNA assemblies can be produced efficiently.