Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Expandable metallic stent therapy for SVC syndrome--effects on local venous pressure, vascular diameter, symptoms, and these correlations].

Nihon Igaku Hoshasen Gakkai zasshi. Nippon acta radiologica·1992
Same author

[Biological effects of static gradient magnetic field on cultured mammalian cells and combined effects with 60Co gamma-rays].

Nihon Igaku Hoshasen Gakkai zasshi. Nippon acta radiologica·1992
Same author

Bleomycin but not its derivatives inhibits the in vivo shedding of a rat tumor-associated antigen.

Anti-cancer drugs·1992
Same author

Isolation of cDNA for bovine stomach 155 kDa protein exhibiting myosin light chain kinase activity.

Journal of biochemistry·1992
Same author

Escherichia coli is able to grow with negligible sodium ion extrusion activity at alkaline pH.

Journal of bacteriology·1992
Same author

Cooperative modulation of voltage-dependent sodium channels by brevetoxin and classical neurotoxins in cultured bovine adrenal medullary cells.

The Journal of pharmacology and experimental therapeutics·1992

Related Experiment Video

Updated: Mar 20, 2026

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture
10:00

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture

Published on: July 20, 2022

2.9K

A pneumatic pressure-driven multi-throughput microfluidic circulation culture system.

T Satoh1, G Narazaki, R Sugita

  • 1Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), Central 5th, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan. shinji.sugiura@aist.go.jp.

Lab on a Chip
|May 28, 2016
PubMed
Summary
This summary is machine-generated.

This study introduces a microfluidic device for multi-throughput cell culture, enabling efficient drug discovery. The system uses pneumatic pressure to circulate medium, enhancing endothelial cell alignment and gene expression under physiological shear stress.

More Related Videos

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device
14:48

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Published on: April 17, 2021

4.6K
A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
11:23

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Published on: October 6, 2019

10.9K

Related Experiment Videos

Last Updated: Mar 20, 2026

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture
10:00

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture

Published on: July 20, 2022

2.9K
Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device
14:48

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Published on: April 17, 2021

4.6K
A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
11:23

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Published on: October 6, 2019

10.9K

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Drug Discovery

Background:

  • Cell culture models are crucial for drug discovery and understanding cellular responses.
  • Physiological shear stress significantly impacts endothelial cell function and gene expression.
  • Existing cell culture systems may lack throughput or precise control over microenvironmental conditions.

Purpose of the Study:

  • To develop a pneumatic pressure-driven microfluidic device for multi-throughput medium circulation culture.
  • To investigate the effects of physiological shear stress on human umbilical vein endothelial cells (HUVECs) in a microfluidic environment.
  • To assess changes in endothelial cell alignment and gene expression under different flow conditions.

Main Methods:

  • A pneumatic pressure-driven microfluidic device with three independent circulation units was designed.
  • Human umbilical vein endothelial cells (HUVECs) were cultured under controlled shear stress conditions.
  • Medium circulation was achieved via programmed pneumatic pressure application.
  • Cell alignment and mRNA expression of specific genes (eNOS, Thrombomodulin) were analyzed.

Main Results:

  • HUVECs exhibited aligned morphology under one-way circulating flow (10 dyn cm⁻² shear stress).
  • Random cell alignment was observed under no shear stress or reciprocating flow.
  • A 2.8- to 4.9-fold increase in eNOS and Thrombomodulin mRNA expression was noted under one-way flow compared to static or reciprocating conditions.

Conclusions:

  • The developed microfluidic device enables multi-throughput circulation culture with precise control over shear stress.
  • Physiological shear stress is critical for inducing endothelial cell alignment and modulating key gene expression.
  • This system offers a valuable platform for drug discovery and studying mechanobiology.