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The effect of miR-340 over-expression on cell-cycle-related genes in triple-negative breast cancer cells

S Mohammadi Yeganeh1,2, M Vasei3, R Tavakoli4

  • 1Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

European Journal of Cancer Care
|May 28, 2016
PubMed

Insights

MicroRNA-340 (miR-340) may suppress triple-negative breast cancer (TNBC) by regulating cell-cycle genes. Overexpressing miR-340 in TNBC cells altered the expression of key genes involved in cell cycle progression and metastasis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Triple-negative breast cancer (TNBC) is aggressive with a high recurrence risk.
  • MicroRNAs (miRNAs) offer potential for targeted TNBC therapies.
  • miR-340 downregulation is observed in metastatic TNBC cells.

Purpose of the Study:

  • To investigate the tumor-suppressive role of miR-340 in TNBC.
  • To determine the effect of miR-340 on cell-cycle-related genes in TNBC.

Main Methods:

  • Overexpression of miR-340 in the MDA-MB-231 TNBC cell line using a lentiviral vector.
  • Quantitative RT-PCR to analyze the expression of cell-cycle regulators (cyclin A2, CDK2, P16, P18, P27, RB) and transcription factors (SMAD 4, SOX2, SOX17).

Main Results:

  • miR-340 overexpression led to decreased expression of SOX2, P16, and P27.
  • miR-340 overexpression resulted in increased expression of cyclin A2, CDK2, SOX17, P18, SMAD 4, and RB.
  • Upregulation of tumor suppressor genes (RB, SOX17) and downregulation of oncogene SOX2 observed.

Conclusions:

  • miR-340 exhibits a tumor-suppressive role in TNBC by modulating cell-cycle gene expression.
  • miR-340 may inhibit breast cancer metastasis, warranting further investigation.
  • This miRNA holds potential as a therapeutic target for TNBC.
Keywords:
cell-cycle genescyclin-dependent kinasecyclin-dependent kinase inhibitormiRNAtriple-negative breast cancer

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