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Improved High-Quality Draft Genome Sequence of Pseudomonas fluorescens KENGFT3
Jennifer Town1, Nina Cui2, Patrice Audy3
1Agriculture and Agri-Food Canada, Saskatoon Research and Development Centre, Saskatoon, Saskatchewan, Canada Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Pseudomonas sp. strain KENGFT3 shows potential as a biopesticide by inhibiting Phytophthora infestans, the cause of potato late blight. Its genome was sequenced, revealing its relation to Pseudomonas fluorescens.
Area of Science:
- Microbiology
- Plant Pathology
- Genomics
Background:
- Pseudomonas sp. strain KENGFT3 exhibits inhibitory effects against Phytophthora infestans.
- Phytophthora infestans is a significant pathogen causing potato late blight.
- The strain KENGFT3 is a candidate for biopesticide development.
Purpose of the Study:
- To investigate the genomic characteristics of Pseudomonas sp. strain KENGFT3.
- To confirm the inhibitory potential of KENGFT3 against Phytophthora infestans.
- To assess the phylogenetic relationship of KENGFT3 with other Pseudomonas species.
Main Methods:
- Whole-genome sequencing of Pseudomonas sp. strain KENGFT3.
- Genome assembly into a single scaffold with 9 contigs.
- Bioinformatic analysis to determine phylogenetic relatedness.
Main Results:
- The genome size of KENGFT3 is 6.2 Mbp.
- The genome was successfully assembled into a single scaffold.
- Phylogenetic analysis indicates KENGFT3 is closely related to Pseudomonas fluorescens.
Conclusions:
- Pseudomonas sp. strain KENGFT3 is a promising biopesticide candidate for managing potato late blight.
- The genomic information provides a foundation for further research and application of KENGFT3.
- Understanding the genetic makeup of KENGFT3 aids in its effective utilization in agriculture.
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