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Related Experiment Video

Updated: Mar 20, 2026

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
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Diagnostic Methods for CHIKV Based on Serological Tools.

Paolo Gaibani1, Maria Poala Landini2,3, Vittorio Sambri4,2

  • 1Regional Reference Centre for Microbiological Emergencies (CRREM), Unit of Microbiology, S. Orsola-Malpighi Hospital, Via G. Massarenti 9-40138, Bologna, Italy. paolo.gaibani@unibo.it.

Methods in Molecular Biology (Clifton, N.J.)
|May 29, 2016
PubMed
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This chapter details serological methods for diagnosing Chikungunya virus (CHIKV) infection. It highlights Enzyme-Linked Immunosorbent Assay (ELISA), Immunofluorescence Assay (IFA), and Microneutralization Assay (MNA) for accurate CHIKV diagnosis and epidemiological studies.

Area of Science:

  • Virology
  • Immunology
  • Infectious Diseases

Background:

  • Chikungunya virus (CHIKV) is a mosquito-borne Alphavirus causing acute febrile illness with severe joint pain, often leading to chronic arthralgia.
  • CHIKV infection is prevalent in tropical and subtropical regions of Africa, Asia, and America.
  • Accurate laboratory diagnosis is crucial for managing CHIKV infections and conducting epidemiological studies.

Purpose of the Study:

  • To present commonly used serological methods for diagnosing Chikungunya virus (CHIKV) infection in humans.
  • To illustrate the principles, interpretation, and procedures of the microneutralization assay (MNA) for CHIKV diagnosis.

Main Methods:

  • Discusses serological diagnostic techniques including Enzyme-Linked Immunosorbent Assay (ELISA) and Immunofluorescence Assay (IFA).
Keywords:
Chikungunya virusELISAIFAMicroneutralizationSerological methods

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  • Explains the role of serum virus neutralization assays as confirmatory tests for CHIKV infection.
  • Details the microneutralization assay (MNA) for determining CHIKV-specific neutralizing antibody titers.

    • Main Results:

    • ELISA and IFA are rapid, sensitive screening tools for CHIKV diagnosis.
    • Serum virus neutralization assays, particularly MNA, offer higher specificity and serve as confirmatory tests.
    • These serological methods are valuable for both individual patient diagnosis and large-scale epidemiological surveillance of CHIKV.

    Conclusions:

    • Serological methods are essential for diagnosing Chikungunya virus infections.
    • A combination of screening assays (ELISA, IFA) and confirmatory assays (MNA) ensures accurate CHIKV diagnosis.
    • Understanding these diagnostic techniques is vital for effective public health strategies against CHIKV.