Related Experiment Video
Updated: Mar 20, 2026
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
Published on: March 13, 2018
Infectious Viral Quantification of Chikungunya Virus-Virus Plaque Assay
Parveen Kaur1, Regina Ching Hua Lee1, Justin Jang Hann Chu2
1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, 5 Science Drive 2, Singapore, 117597, Singapore.
Abstract:
The plaque assay is an essential method for quantification of infectious virus titer. Cells infected with virus particles are overlaid with a viscous substrate. A suitable incubation period results in the formation of plaques, which can be fixed and stained for visualization. Here, we describe a method for measuring Chikungunya virus (CHIKV) titers via virus plaque assays.
