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Virus Isolation and Preparation of Sucrose-Banded Chikungunya Virus Samples for Transmission Electron Microscopy
1Laboratory of Neurovirology, Department of Virology 1, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuiku-ku, Tokyo, 162-8640, Japan. ck@nih.go.jp.
Methods in Molecular Biology (Clifton, N.J.)
|May 29, 2016
Abstract:
Virus isolation and purification is an invaluable technique in virology to detect and characterize viruses. This chapter describes a large-scale Chikungunya virus (CHIKV) propagation and purification methods by using discontinuous sucrose gradient, and sample preparation for transmission electron microscopy. Sucrose-banding yields large quantities of high-titer (10(10) pfu/ml) CHIKV stocks. Such stocks are stable for years when stored at -70 °C.
