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Viral-Host Protein Interaction Studies Using Yeast Two-Hybrid Screening Method.

Namrata Dudha¹, Sanjay Gupta²

¹Centre for Emerging Diseases, Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector 62, Noida, 201307, Uttar Pradesh, India.

Methods in Molecular Biology (Clifton, N.J.)

|May 29, 2016

Summary

This summary is machine-generated.

The Yeast two-hybrid (Y2H) assay is a key method for mapping protein interactions. This chapter details using Y2H to study viral-host interactions, focusing on Chikungunya virus (CHIKV).

Keywords:

Chikungunya virus Human cDNA library Protein–protein interactions Virus–host interactions Yeast two-hybrid

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Last Updated: Mar 20, 2026

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A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

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Published on: July 18, 2013

Area of Science:

Molecular Biology
Virology
Proteomics

Background:

Protein-protein interactions are crucial for understanding biological processes.
Viruses rely on host cell machinery for replication, making viral-host interactions vital for infection.
The Yeast two-hybrid (Y2H) assay is a foundational technique for identifying protein interactions.

Purpose of the Study:

To provide a detailed explanation of the Y2H assay.
To illustrate the application of Y2H for studying viral-host protein interactions.
To focus on the Chikungunya virus (CHIKV) and its host interactions.

Main Methods:

Detailed explanation of the Yeast two-hybrid (Y2H) assay methodology.
Application of Y2H for identifying protein interactions between viral and host proteins.
Specific focus on interactions involving Chikungunya virus (CHIKV).

Main Results:

The Y2H assay has established itself as a valuable tool in the proteomics era.
Y2H enables the creation of protein interaction maps for various organisms.
This chapter will detail the Y2H assay for studying CHIKV-host interactions.

Conclusions:

Understanding viral-host interactions is essential for comprehending viral life cycles.
Identifying key interaction players can lead to strategies for preventing pathogenesis.
The Y2H assay is a powerful method for dissecting viral-host protein interactions.