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Updated: Mar 20, 2026
High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
Published on: May 5, 2014
Using Bicistronic Baculovirus Expression Vector System to Screen the Compounds That Interfere with the Infection of
Szu-Cheng Kuo1, Chao-Yi Teng2, Yi-Jung Ho1
1Institute of Prevention Medicine, National Defense Medical Center, Taipei, Taiwan.
Abstract:
Chikungunya virus (CHIKV) is the etiologic agent of Chikungunya fever and has emerged in many countries over the past decade. There are no effective drugs for controlling the disease. A bicistronic baculovirus expression system was utilized to co-express CHIKV structural proteins C (capsid), E2 and E1 and the enhanced green fluorescence protein (EGFP) in Spodoptera frugiperda insect cells (Sf21). The EGFP-positive Sf21 cells fused with each other and with uninfected cells to form a syncytium is mediated by the CHIKV E1 allowing it to identify chemicals that can prevent syncytium formation. The compounds characterized by this method could be anti-CHIKV drugs.
