Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tephritid26: A standardized, multi-angle image dataset of quarantine-significant true fruit flies for deep learning-based identification.

Scientific data·2026
Same author

Prion propagation is controlled by discrete structural regions of PrP rather than overall stability.

The Journal of biological chemistry·2026
Same author

Dose-dependent effects of gamma-ray irradiation on SLA-treated titanium Grade 4: An in vitro evaluation of its physical, chemical and surface properties.

Dental and medical problems·2026
Same author

Clinical and Esthetic Outcomes of Anodized Titanium Abutments: A Systematic Review of Randomized Controlled Trials.

Clinical, cosmetic and investigational dentistry·2025
Same author

In Vivo Irritation and Sensitization Assessment of Cypermethrin, Pymetrozine, and Indoxacarb-Emamectin Benzoate Combination in Test Animals.

Journal of toxicology·2025
Same author

Biodegradation, Angiogenesis, and Inflammatory Response of a Collagen-Chitosan-Polyvinyl Alcohol (PVA) Membrane: In Vivo Model of Guided Tissue Regeneration.

European journal of dentistry·2025

Related Experiment Video

Updated: Mar 20, 2026

Author Spotlight: Sieving Fruit Pulp to Detect Immature Tephritid Fruit Flies in the Field
04:21

Author Spotlight: Sieving Fruit Pulp to Detect Immature Tephritid Fruit Flies in the Field

Published on: July 28, 2023

2.6K

A high-throughput detection method for invasive fruit fly (Diptera: Tephritidae) species based on microfluidic

Fan Jiang1,2, Wei Fu2, Anthony R Clarke3

  • 1College of Plant Protection, China Agricultural University, Beijing, 100193, China.

Molecular Ecology Resources
|May 29, 2016
PubMed
Summary

Rapid DNA diagnostics for invasive fruit flies are crucial. This new method uses qPCR on microfluidic arrays for fast, accurate identification of 27 tephritidae species, aiding plant quarantine efforts.

Keywords:
DNA barcodesFluidigm systembiological invasionmolecular identificationplant quarantinespecies-specific

More Related Videos

A High Throughput Microplate Feeder Assay for Quantification of Consumption in Drosophila
08:55

A High Throughput Microplate Feeder Assay for Quantification of Consumption in Drosophila

Published on: June 14, 2021

3.9K
Assessing the Cellular Immune Response of the Fruit Fly, Drosophila melanogaster, Using an In Vivo Phagocytosis Assay
05:50

Assessing the Cellular Immune Response of the Fruit Fly, Drosophila melanogaster, Using an In Vivo Phagocytosis Assay

Published on: April 10, 2019

8.1K

Related Experiment Videos

Last Updated: Mar 20, 2026

Author Spotlight: Sieving Fruit Pulp to Detect Immature Tephritid Fruit Flies in the Field
04:21

Author Spotlight: Sieving Fruit Pulp to Detect Immature Tephritid Fruit Flies in the Field

Published on: July 28, 2023

2.6K
A High Throughput Microplate Feeder Assay for Quantification of Consumption in Drosophila
08:55

A High Throughput Microplate Feeder Assay for Quantification of Consumption in Drosophila

Published on: June 14, 2021

3.9K
Assessing the Cellular Immune Response of the Fruit Fly, Drosophila melanogaster, Using an In Vivo Phagocytosis Assay
05:50

Assessing the Cellular Immune Response of the Fruit Fly, Drosophila melanogaster, Using an In Vivo Phagocytosis Assay

Published on: April 10, 2019

8.1K

Area of Science:

  • Molecular Biology
  • Entomology
  • Invasive Species Management

Background:

  • Invasive species pose significant ecological, economic, and health risks.
  • High-throughput, DNA-based diagnostics are needed for rapid identification of exotic organisms.
  • Limited taxonomic expertise hinders timely detection of biological invasions.

Purpose of the Study:

  • To develop species-specific, high-throughput DNA diagnostics for 27 economically important tephritidae species.
  • To create a standardized microfluidic system for simultaneous detection of multiple fruit fly species.
  • To validate the method using unknown immature samples from quarantine interceptions and field monitoring.

Main Methods:

  • Designed species-specific qPCR (quantitative polymerase chain reaction) primer and probe combinations based on COI DNA barcode haplotypes.
  • Tested primer/probe specificity using qPCR on 35 tephritidae species.
  • Developed a microfluidic dynamic array system for simultaneous detection of 27 target species.

Main Results:

  • Achieved specific and simultaneous detection of all 27 target tephritidae species within 7.5 hours.
  • The method successfully discriminated among species with up to 98.48% genetic similarity.
  • Identified unknown immature samples accurately, consistent with adult morphological identification.

Conclusions:

  • An accurate, rapid, and high-throughput qPCR-based microfluidic approach for fruit fly quarantine detection was developed.
  • This innovative method demonstrates broad potential for international plant quarantine and invasive species detection standards.
  • The system enables efficient identification of invasive fruit flies, crucial for biosecurity.