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A specific androgen-binding protein (ABP) in Necturus testis and its zonal distribution
1Department of Biology, Boston University, Massachusetts 02215.
The Journal of Experimental Zoology
|April 1, 1989
Summary
Researchers identified a novel androgen-binding protein (ABP) in the Necturus maculosus testis. This protein, distinct from the androgen receptor, shows specific binding to androgens and is differentially distributed within the testis.
Area of Science:
- Reproductive Biology
- Endocrinology
- Amphibian Physiology
Background:
- The zoned testis of Necturus maculosus offers a unique model for studying stage-dependent biochemical changes.
- Separation of Leydig cells from germinal elements facilitates targeted investigations.
Purpose of the Study:
- To identify and characterize androgen-binding proteins in the Necturus maculosus testis.
- To differentiate these proteins from the known androgen receptor.
Main Methods:
- Standard binding assays using [3H]testosterone (T).
- Dextran-coated charcoal (DCC) and Sephadex LH-20 for steroid separation.
- Sucrose gradient centrifugation to determine sedimentation properties.
Main Results:
- An androgen-binding protein (ABP) with high affinity (Kd = 10(-9) M) and specificity for androgens (T, DHT) was identified.
- This ABP differs from the androgen receptor by its abundance, rapid dissociation rate, and lack of DNA-cellulose adherence or nuclear presence.
- The protein sediments at 6-7 S and shows differential distribution within the testis, with higher levels in immature regions.
Conclusions:
- A distinct androgen-binding protein, resembling a serum sex steroid-binding protein, exists in the Necturus testis.
- Its cellular source and precise function within the testis require further investigation.
