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Related Experiment Videos

Cysteine: depolarization-induced release from rat brain in vitro.

H J Keller1, K Q Do, M Zollinger

  • 1Brain Research Institute, University of Zürich, Switzerland.

Journal of Neurochemistry
|June 1, 1989
PubMed
Summary

Researchers identified cysteine (Cys) as a neuroactive substance released from rat brain slices. This discovery enables a new method for quantifying Cys, suggesting its role in neurotransmission.

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Area of Science:

  • Neuroscience
  • Biochemistry

Background:

  • Depolarization of neurons releases various compounds.
  • Identifying neuroactive substances is crucial for understanding brain function.

Purpose of the Study:

  • To identify novel neuroactive substances released from rat brain slices.
  • To investigate the role of cysteine in neurotransmission.

Main Methods:

  • Rat brain slices were depolarized to collect superfusates.
  • Compounds were analyzed using reversed-phase HPLC and mass spectrometry.
  • A new quantification method for cysteine was developed using N-ethylmaleimide.

Main Results:

  • S-carboxamidomethylcysteine, a stable cysteine derivative, was identified.

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  • Extracellular cysteine levels increased upon depolarization.
  • Cysteine release was most prominent in the neocortex and mesodiencephalon.

    • Conclusions:

    • Cysteine is released in a calcium-dependent manner from neurons.
    • Cysteine may function as a neurotransmitter or neuromodulator.
    • The developed method allows for accurate cysteine quantification in brain tissue.