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Updated: Mar 20, 2026
Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones
Published on: February 27, 2021
Soil-borne wheat mosaic virus infectious clone and manipulation for gene-carrying capacity
Sridhar Jarugula1,2, Steven R Charlesworth1,3, Feng Qu1,2
1Center for Applied Plant Sciences, Ohio State University, Ohio Agriculture Research and Development Center (OARDC), Wooster, OH, USA.
Abstract:
A full-length infectious cDNA clone of soil-borne wheat mosaic virus (SBWMV; genus Furovirus; family Virgaviridae) was developed for agrobacterium delivery. The cloned virus can be agroinfiltrated to Nicotiana benthamiana for subsequent infection of wheat (Triticum aestivum, L.). The utility of the virus as a vector for gene silencing and expression was assessed through sequence insertions in multiple sites of RNA2. Virus-induced photobleaching was observed in N. benthamiana but not in wheat, despite the stability of the inserts. The SBWMV infectious clone can be used for further studies to investigate the biology of SBWMV through mutagenesis.
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