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Complete genome sequence of a coxsackievirus B3 recombinant isolated from an aseptic meningitis outbreak in eastern

Wenqiang Zhang1,2, Xiaojuan Lin1,2, Ping Jiang3

  • 1Academy of Preventive Medicine, Shandong University, Jinan, People's Republic of China.

Archives of Virology
|May 30, 2016
PubMed

Insights

Coxsackievirus B3 (CV-B3) strains in China show genetic recombination and mutation, contributing to aseptic meningitis. Further surveillance is crucial due to co-circulation in mainland China and Hong Kong.

Area of Science:

  • Virology
  • Molecular Biology
  • Epidemiology

Background:

  • Coxsackievirus B3 (CV-B3) is a significant cause of aseptic meningitis outbreaks.
  • Previous studies have linked CV-B3 to neurological diseases, but specific genetic drivers remain under investigation.
  • Understanding viral genetic diversity is key to controlling outbreaks and predicting disease severity.

Purpose of the Study:

  • To identify sequence motifs associated with aseptic meningitis caused by CV-B3.
  • To construct an infectious clone of a representative CV-B3 strain.
  • To analyze the genetic relatedness and evolutionary patterns of CV-B3 strains in China.

Main Methods:

  • Whole genome sequencing of the 08TC170 CV-B3 strain isolated from cerebrospinal fluid.
  • Sequence alignment and phylogenetic analysis of coding regions (P1-P3, VP1).
  • Similarity plot analysis to identify potential recombination events.
  • Mutation analysis, specifically focusing on the T277A substitution.

Main Results:

  • The 08TC170 strain shares high sequence identity (≥97.7%) with other Chinese CV-B3 strains, forming a distinct cluster.
  • Evidence of intertypic recombination was found, with similarity to CV-B5 or E-6 strains in specific genomic regions.
  • A novel T277A mutation was identified in strains from 2008-2010, distinguishing them from earlier isolates.

Conclusions:

  • The 08TC170 CV-B3 strain likely resulted from both intertypic recombination and point mutation.
  • The identified genetic features suggest co-circulation of related CV-B3 strains in mainland China and Hong Kong.
  • Ongoing molecular surveillance is recommended to monitor CV-B3 evolution and potential neurovirulence.

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