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Updated: Mar 20, 2026
Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model
Published on: March 20, 2020
[Antitumor Effect of Ganoderma lipsiense Extract on Triple-negative Breast Cancer Model Mice and Mechanism Study]
Objective:
To study the inhibitory effect and mechanism of Ganoderma lipsiense extract (GLE) on the growth of triple-negative breast cancer (TNBC) cell line MDA-MB-231-HM in a mouse model.
Methods:
The mouse model of TNBC was established by subcutaneous injection of 1.5 x 10(6) of MDA-MB-231-HM cells into BALB/c-nu mouse. Twenty successfully modeled mice were divided into the GLE group and the negative control group according to random digit table, 10 in each group. GLE (0.2 mL 100 mg/mL) was peritoneally injected to mice in the GLE group, while equal dose of normal saline was peritoneally injected to mice in the negative control group. The medication was administered once per 3 days and discontinued after 45 days. The CD34 expression was detected using immunohistochemical assay for counting microvessels. Meanwhile, expressions of thrombospondin 1 (TSP-1) and cyclin D1 were detected using immunohistochemical assay.
Results:
The average weight was obviously lower in the GLE group than in the negative control group [(0.33 ± 0.16) g vs (0.68 ± 0.37)g, P < 0.05]. The tumor inhibition rate was 51.4% in the GLE group. The volume of transplanted tumor was obviously lesser in the GLE group than in the negative control group (P < 0.05). Results of immunohistochemical staining showed, the microvessel density (MVD) under every field was (20.7 ± 2.1), TSP-1 positive cell count was (66.2 ± 9.2), cyclin D1 positive cell count was (33.8 ± 16.4) in the GLE group, and they were 34.0 ± 2.0, 24.0 ± 6.6, and 168.2 ± 32.6, respectively in the negative control group. There was statistical difference in all indices between the two groups (P < 0.05).
Conclusion:
GLE could inhibit malignant proliferation of tumor cells by suppressing angiogenesis of blood vessels in tumor tissues and regulating cell cycles, thereby inhibiting TNBC.
Insights
Ganoderma lipsiense extract (GLE) significantly inhibited triple-negative breast cancer (TNBC) growth in mice by reducing tumor weight and volume. GLE suppressed angiogenesis and regulated cell cycle-related proteins, offering a potential therapeutic strategy for TNBC.
Area of Science:
- Oncology
- Pharmacology
- Biomedical Research
Background:
- Triple-negative breast cancer (TNBC) is an aggressive subtype with limited treatment options.
- Identifying novel therapeutic agents for TNBC is a critical unmet need.
- Ganoderma lipsiense extract (GLE) has demonstrated various biological activities.
Purpose of the Study:
- To investigate the anti-cancer effects of Ganoderma lipsiense extract (GLE) on triple-negative breast cancer (TNBC) cell line MDA-MB-231-HM.
- To elucidate the underlying mechanisms of GLE's inhibitory action in a mouse model.
Main Methods:
- A TNBC mouse model was established using MDA-MB-231-HM cells.
- Mice were treated with GLE or a negative control (normal saline).
- Tumor growth, microvessel density (MVD), and expressions of thrombospondin 1 (TSP-1) and cyclin D1 were assessed using immunohistochemistry.
Main Results:
- GLE treatment resulted in a 51.4% tumor inhibition rate, significantly reducing tumor weight and volume compared to controls.
- GLE significantly decreased MVD and the positive cell counts for TSP-1 and cyclin D1.
- These findings indicate GLE's potent anti-angiogenic and anti-proliferative effects.
Conclusions:
- Ganoderma lipsiense extract (GLE) exhibits significant inhibitory effects on TNBC growth.
- GLE suppresses tumor progression by inhibiting angiogenesis and regulating cell cycle-related proteins.
- GLE represents a promising natural compound for TNBC therapy.
