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Related Concept Videos

Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

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For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
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Ligand Binding Sites02:40

Ligand Binding Sites

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Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
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Conserved Binding Sites01:49

Conserved Binding Sites

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Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
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Ligand Binding and Linkage00:49

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Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
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Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Metal-Ligand Bonds02:51

Metal-Ligand Bonds

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The hemoglobin in the blood, the chlorophyll in green plants, vitamin B-12, and the catalyst used in the manufacture of polyethylene all contain coordination compounds. Ions of the metals, especially the transition metals, are likely to form complexes.
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Analyzing and Building Nucleic Acid Structures with 3DNA
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Structure-Specific Ligand Recognition of Multistranded DNA Structures.

Mahima Kaushik1, Anju Singh, Mohan Kumar

  • 1Cluster Innovation Centre, University of Delhi, Delhi, India.

Current Topics in Medicinal Chemistry
|May 31, 2016
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This summary is machine-generated.

Nucleic acids exhibit structural polymorphism, forming diverse DNA and RNA structures that interact with drugs. These interactions are crucial for developing targeted therapies with anticancer and antimicrobial properties.

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Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Medicinal Chemistry

Background:

  • Nucleic acids, including DNA and RNA, display structural polymorphism, adopting various canonical, alternative, and multistranded conformations.
  • These alternative nucleic acid structures can interact with ligands like proteins, drugs, and metal ions in a sequence- and structure-specific manner.
  • DNA-ligand interactions are therapeutically relevant, with many DNA/RNA-targeting drugs exhibiting anticancer, antibacterial, anthelmintic, and antiviral activities.

Purpose of the Study:

  • To review structural polymorphs of DNA and RNA.
  • To explain the interactions between these structures and pharmaceutical drugs.
  • To discuss the biological relevance and therapeutic potential of these interactions.

Main Methods:

  • Literature review of structural polymorphism in nucleic acids.
  • Analysis of DNA-drug interactions and their mechanisms.
  • Exploration of therapeutic applications and drug development strategies.

Main Results:

  • Structural polymorphism in nucleic acids enables specific recognition of ligands, including therapeutic drugs.
  • DNA-drug interactions are key to developing drugs with diverse pharmacological activities.
  • Understanding these interactions aids in designing novel drugs with enhanced efficacy and specificity.

Conclusions:

  • Multistranded DNA structures and their interactions with drugs offer significant potential for therapeutic applications.
  • Further research into these molecular targets can facilitate precise drug development.
  • Exploiting structural polymorphism in nucleic acids is vital for advancing medicinal chemistry and drug discovery.