Related Experiment Video
Updated: Mar 20, 2026
10:10
Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
Published on: February 16, 2017
23.0K
miR-145a-5p Promotes Myoblast Differentiation.
Jingjing Du1, Qiang Li2, Linyuan Shen1
1College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Biomed Research International
|May 31, 2016
Summary
MicroRNAs regulate gene expression. This study shows microRNA-145a-5p promotes C2C12 myoblast differentiation by enhancing key myogenic and Wnt signaling genes.
Area of Science:
- Molecular Biology
- Cell Biology
Background:
- MicroRNAs are noncoding RNAs regulating gene expression.
- MicroRNAs play crucial roles in myoblast differentiation.
Purpose of the Study:
- To investigate the role of miR-145a-5p in C2C12 myoblast differentiation.
- To explore the impact of miR-145a-5p on myogenic and Wnt signaling pathways.
Main Methods:
- Monitoring miR-145a-5p expression during C2C12 cell differentiation.
- Utilizing miR-145a-5p inhibitors and mimics to assess gene expression changes.
- Analyzing the effects on myogenesis marker genes and Wnt signaling components.
Main Results:
- miR-145a-5p expression increased during C2C12 myoblast differentiation.
- miR-145a-5p manipulation altered expression of myogenic marker genes.
- miR-145a-5p influenced key Wnt signaling genes (Wnt5a, LRP5, Axin2, β-catenin).
Conclusions:
- miR-145a-5p acts as a positive regulator of C2C12 myoblast differentiation.
- miR-145a-5p promotes differentiation by enhancing myogenic and Wnt signaling pathways.
Related Concept Videos
01:13Formation of Muscle Fibers from Myoblasts
6.7K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
6.7K
01:21Satellite Stem Cells and Muscular Dystrophy
2.5K
Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
2.5K
02:23Master Transcription Regulators
8.0K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
8.0K