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Related Experiment Video

Updated: Mar 20, 2026

Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds

Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds

Published on: May 5, 2016

Generation of Mouse Lung Epithelial Cells.

Andrea L Kasinski¹, Frank J Slack¹

¹Molecular, Cellular and Developmental Biology Department, Yale University, New Haven, USA.

|May 31, 2016

Summary

This study details a method for generating lung epithelial cells from Kras mice. This technique facilitates in vitro study of lung adenocarcinoma development and cellular processes.

Area of Science:

* Mammalian cell culture and molecular biology.
* Cancer research and in vitro modeling.

Background:

* In vivo models are limited for studying cellular and molecular events.
* In vitro cell culture offers a refined and cost-effective alternative.
* Generating specific cell lines from animal models is crucial for research.

Purpose of the Study:

* To describe a protocol for generating lung epithelial cells from Kras mice.
* To establish a cell line for studying lung adenocarcinoma in vitro.
* To provide a method applicable to various genetic backgrounds.

Main Methods:

* Generation of lung epithelial cells from Kras mice.
* Utilizing cre-recombinase dependent genetic manipulation.
* Culture and characterization of the resulting cell line.

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Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching

Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching

Published on: June 30, 2010

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease

Published on: April 12, 2021

Related Experiment Videos

Last Updated: Mar 20, 2026

Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds

Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds

Published on: May 5, 2016

Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching

Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching

Published on: June 30, 2010

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease

Published on: April 12, 2021

Main Results:

* Successful generation of lung epithelial cells from Kras mice.
* The Kras; p53 cell line proliferates in culture without further genetic modification.
* Established a model for studying aggressive lung adenocarcinoma.

Conclusions:

* The described protocol enables the generation of lung epithelial cell lines from Kras mice.
* This method provides a valuable tool for in vitro cancer research.
* The Kras; p53 cell line is a robust model for studying lung adenocarcinoma progression.