Pt@AuNPs integrated quantitative capillary-based biosensors for point-of-care testing application
Ze Wu1, Qiangqiang Fu1, Shiting Yu1
1Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou 510632, PR China.
Biosensors & Bioelectronics
|May 31, 2016
Summary
A new portable, cost-effective capillary biosensor enables naked-eye detection of biomarkers like prostate-specific antigen (PSA) and carcinoembryonic antigen (CEA) for point-of-care testing.
Area of Science:
- Nanotechnology
- Biomedical Engineering
- Analytical Chemistry
Background:
- Current diagnostic tools are often bulky and expensive, limiting their use in point-of-care settings.
- There is a significant need for portable, cost-effective diagnostic solutions for rapid biomarker detection.
- Developing simple, visual detection methods is crucial for accessible healthcare.
Purpose of the Study:
- To develop and validate a cost-effective, portable capillary-based biosensor for quantitative biomarker detection.
- To demonstrate the biosensor's capability for naked-eye readout using visual cues.
- To assess the biosensor's performance in detecting cancer biomarkers like PSA and CEA.
Main Methods:
- Utilized core-shell Pt@Au nanoparticles (Pt@AuNPs) as catalysts to decompose hydrogen peroxide (H2O2), producing oxygen (O2).
- Developed a capillary-based biosensor where O2 production, indicated by blue ink movement, correlates with biomarker concentration.
- Constructed sandwich immunoassays by linking Pt@AuNPs with antibodies for specific protein capture.
Main Results:
- The distance of ink movement in the capillary was directly proportional to the concentration of target proteins.
- Achieved a linear detection range (LDR) of 0.02–2.5 ng/mL for prostate-specific antigen (PSA) with a limit of detection (LOD) of 0.017 ng/mL.
- Demonstrated an LDR of 0.063–16 ng/mL for carcinoembryonic antigen (CEA) with an LOD of 0.044 ng/mL.
- Results from clinical serum samples showed good correlation with established chemiluminescence immunoassays (CLIAs).
Conclusions:
- The developed capillary-based biosensor offers a cost-effective and portable solution for quantitative biomarker detection.
- The naked-eye readout mechanism simplifies the diagnostic process, making it suitable for point-of-care applications.
- This biosensor technology shows promise for accessible and rapid disease screening, particularly for cancer biomarkers.
