Transcriptome and Difference Analysis of Fenpropathrin Resistant Predatory Mite, Neoseiulus barkeri (Hughes)

Area of Science:

• Entomology
• Molecular Biology
• Biochemistry

Background:

• Fenpropathrin resistance is increasing in predatory mites.
• The molecular mechanisms underlying this resistance are not well understood.
• Neoseiulus barkeri is an important predatory mite in agriculture.

Purpose of the Study:

• To investigate the molecular basis of fenpropathrin resistance in Neoseiulus barkeri.
• To identify potential resistance-related genes and mutations.
• To provide insights into the genetic mechanisms of pesticide resistance in predatory mites.

Main Methods:

• Transcriptome sequencing of Neoseiulus barkeri using Illumina platform.
• Manual annotation of unigenes to identify resistance-related genes.
• Polymorphism analysis and gene expression quantification (qPCR).

Main Results:

• 34,211 unigenes were obtained, with 15,987 manually annotated.
• Two point mutations were identified in the voltage-gated sodium channel (VGSC) gene.
• 43 cytochrome P450s (P450s) and 10 glutathione S-transferases (GSTs) were identified, with significant overexpression of specific P450 and GST genes in resistant strains.

Conclusions:

• Fenpropathrin resistance in N. barkeri is a complex trait involving multiple genetic alterations.
• Overexpression of specific P450 and GST genes contributes to fenpropathrin resistance.
• This study provides a foundation for understanding and managing pesticide resistance in predatory mites.